Batduck (Daffy) and his sidekicks (Porky and Petunia) have once again to save Gotham City from the evil forces.
- Daffy Duck (as Batduck, voiced by Jeff Bergman)
- Porky Pig (as Ropig, voiced by Bob Bergen)
- Petunia Pig (as Batpigirl, voiced by Grey DeLisle)
- Yosemite Sam (as The Yosemite Joker, voiced by Maurice LaMarche)
- Gabby Goat (as Alfred Gabbyworth, voiced by Rob Paulsen)
- Sam Sheepdog (as Commissioner Sam Gordon, voiced by Jim Cummings)
- "I hate this job" - Gabby Goat.
- "Well, my suine sidekicks, being a superhero isn't an easy job" - Daffy Duck.
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